Myf5基因在马身猪和大白猪背最长肌中的发育性表达研究

doi:10.11843/j.issn.0366-6964.2014.12.024

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (12): 2081-2087.doi: 10.11843/j.issn.0366-6964.2014.12.024

Myf5基因在马身猪和大白猪背最长肌中的发育性表达研究

黎威¹，高鹏飞¹，石建中²，张燕青¹，徐凯¹，刘宏²，郭晓红¹，李步高¹，曹果清^1*

（1.山西农业大学动物科技学院，太谷 030801； 2.大同市种猪场，大同 037000）

收稿日期:2014-04-17 出版日期:2014-12-23 发布日期:2014-12-23
通讯作者: 曹果清，博士，教授，主要从事动物遗传育种与繁殖方面的研究，E-mail:anniecao710502@aliyun.com
作者简介:黎威（1988-），男，湖北石首人，硕士生，主要从事分子遗传学与动物育种研究，E-mail：qq798983696@126.com；高鹏飞（1980-），男，山西太谷人，博士，副教授，主要从事动物遗传育种与繁殖方面的研究，E-mail:gpf800411@126.com。黎威和高鹏飞同为第一作者
基金资助:
山西省科技攻关项目（20140311020-5；20080311031）；山西省留学人员科技活动择优资助项目（614193）；山西省高等学校优秀青年学术带头人支持计划；山西农业大学“中青年学术带头人及学术骨干”支持计划

Study on the Developmental Expression of Myf5 Gene in Longissimus Dorsi Tissue of Mashen and Large White Pigs

LI Wei¹，GAO Peng-fei¹，SHI Jian-zhong²，ZHANG Yan-qing¹，XU Kai¹，LIU Hong²，GUO Xiao-hong¹，LI Bu-gao¹，CAO Guo-qing^1*

(1.College of Animal Science and Veterinary Medicine，Shanxi Agricultural University，Taigu 030801，China；2.Datong Pig Breeding Farm，Datong 037000，China)

Received:2014-04-17 Online:2014-12-23 Published:2014-12-23

摘要/Abstract

摘要：

本研究旨在分析猪背最长肌中Myf5基因的发育性表达规律。采用实时荧光定量PCR和Western-blot技术检测了马身猪和大白猪从初生~180日龄7个阶段（初生、30、60、90、120、150和180 d）背最长肌中Myf5基因mRNA和蛋白质发育性表达规律。结果表明，马身猪和大白猪背最长肌中Myf5基因mRNA相对表达量均表现出初生时最高，30日龄次之，60日龄时突然降低，此后基本维持稳定状态的规律。马身猪背最长肌中Myf5蛋白的相对表达量初生时最高，30日龄时最低，60～180日龄阶段，表达量呈现波动变化的不稳定趋势；在大白猪中，Myf5蛋白60日龄前的相对表达量极显著高于60日龄后的表达量（P<0.05），60日龄前各阶段间的表达量及60日龄后各阶段间的表达量差异不显著（P>0.05）。在同一日龄阶段，马身猪Myf5蛋白的表达量均显著或极显著地高于大白猪（60日龄阶段除外）。Myf5在mRNA和蛋白质水平上的表达与猪的遗传背景和发育阶段有关。

Abstract:

The aim of this study was to investigate the expression patterns of Myf5 gene at the levels of mRNA and protein.The expression patterns of Myf5 gene at the levels of mRNA and protein in longissimus dorsi at 7 developmental stages (1-，30-，60-，90-,120-，150-，and 180-day-old) of Mashen and Large White pigs were studied by quantitative real-time PCR and Western-blot.The results showed that the highest mRNA relative expression of Myf5 gene in longissimus dorsi was at 1-day-old，then at 30-day old both in Mashen and Large White pigs.The mRNA relative expression of Myf5 gene decreased sharply at 60-day-old，then keep stable lower levels afterwards.The expression of Myf5 protein in longissimus dorsi of Mashen pig at 1-day-old was the highest and the lowest at 30-day-old.During the stages from 60- to 180-day-old，the relative expression of Myf5 peotein in Mashen pig was in waves with trend of increase and decrease.In Large White pig，the relative expressions of Myf5 protein at the stages before 60-day-old were significant greater than those at the stages after 60-day-old，wherease the expressions at detected stages before 60-day-old or after 60-day-old were not significant different.At the same stages，the relative expression of Myf5 protein in Mashen pig was always greater than that in Large White pig at all detectd stages apart from that at 60-day-old.The expression patterns of Myf5 at the levels of mRNA and protein were associated with the genetic background and the developmental stages of invididuals.

中图分类号:

黎威，高鹏飞，石建中，张燕青，徐凯，刘宏,郭晓红，李步高，曹果清. Myf5基因在马身猪和大白猪背最长肌中的发育性表达研究[J]. 畜牧兽医学报, 2014, 45(12): 2081-2087.

LI Wei，GAO Peng-fei，SHI Jian-zhong，ZHANG Yan-qing，XU Kai，LIU Hong，GUO Xiao-hong，LI Bu-gao，CAO Guo-qing. Study on the Developmental Expression of Myf5 Gene in Longissimus Dorsi Tissue of Mashen and Large White Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 2081-2087.

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Myf5基因在马身猪和大白猪背最长肌中的发育性表达研究

Study on the Developmental Expression of Myf5 Gene in Longissimus Dorsi Tissue of Mashen and Large White Pigs

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